BCL2 Antibody

SKU: orb1251891

Description

BCL2 Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: FC, IF, IHC, WB
• Reactivity: Human
• Application Notes:
  Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 µg/mLWestern blot: 0.5-1 µg/mLImmunohistochemistry (FFPE): 0.5-1 µg/mL for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The optimal dilution of the anti-Bcl-2 antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone No.: SPM530
• Immunogen: Amino acids 41-54 (GAAPAPGIFSSQPG) from the human protein were used as the immunogen for this anti-Bcl-2 antibody.
• Target: BCL2
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Bcl-2 Antibody: Bcl-2, PPP1R50, Apoptosis regulator Bcl-2

Formalin-fixed, paraffin-embedded human non-Hodgkin's lymphoma stained with anti-Bcl-2 antibody (SPM530).

Western blot of Bcl-2 in human skin using anti-Bcl-2 antibody (SPM530).

Quick Database Links

Gene Symbol

BCL2

UniProt

P10415