Bcl2 Antibody

SKU: orb750150

Description

Bcl2 suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release. [UniProt]

Research Area

Apoptotic, Cancer, Epigenetics, Neuroscience, Signaling Pathways

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
• Reactivity: Human
• Application Notes:
  Optimal dilution of the Bcl2 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: ARBC2-1
• Immunogen: Recombinant full-length human protein was used as the immunogen for the Bcl2 antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

IHC testing of FFPE human melanoma with Bcl2 antibody (clone ARBC2-1). HIER: boil tissue sections in 10 mM Tris with 1 mM EDTA, pH9, for 10-20 min and allow to cool before testing.

IHC testing of FFPE human tonsil with Bcl2 antibody (clone ARBC2-1). HIER: boil tissue sections in 10 mM Tris with 1 mM EDTA, pH9, for 10-20 min and allow to cool before testing.

Western blot testing of human MCF7 cell lysate with Bcl2 antibody (clone ARBC2-1). Routinely observed at 18-22 kDa.

Quick Database Links

UniProt

P10415