Bcl-2 Antibody [124]

• Catalog Number: orb1252824
• Category: Antibodies
• Description: Bcl-2 Antibody [124]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 124
• Tested applications: ELISA, FC, IF, IHC, WB
• Reactivity: Human
• Isotype: IgG1, kappa
• Immunogen: A synthetic peptide, aa 41-54 (GAAPAPGIFSSQPG-Cys) of human Bcl-2 protein was used as the immunogen for this antibody.
• Antibody Type: Primary Antibody
• Concentration: 0.2 mg/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: BCL2
• UniProt ID: P10415
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: Bcl-2 Antibody: Bcl-2, PPP1R50, Apoptosis regulato
• Note: For research use only
• Application notes: ELISA: order BSA-free format for coatingFlow Cytometry: 0.5-1 ug/million cellsIF: 0.5-1 ug/mlWB: 0.5-1 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 1mM EDTA Buffer, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of human non-Hodgkin's lymphoma stained with Bcl-2 antibody (124). Note nuclear membrane & cytoplasmic staining.

Western blot testing of human samples using Bcl-2 antibody (124).

FACS staining (intracellular) of Jurkat cells using Bcl-2 antibody (clone 124, red) and isotype control antibody (green).