Bax Rabbit Polyclonal Antibody

• Catalog Number: orb312174
• Category: Antibodies
• Description: Bax Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Porcine, Sheep
• Reactivity: Human, Mouse, Rabbit, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Bax (151-190/190aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100, IF=1:100-500, Flow-Cyt=1μg /test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 21 kDa
• Target: BAX
• UniProt ID: Q07812
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Preservative: 0.02% Proclin300, Constituents: 1% rAlbumin, 0.01M PBS, pH 7.4.
• Alternative names: apoptosis regulator BAX; Apoptosis regulator BAX c
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Gastal, G. D. A. et al. Equine ovarian tissue viability after cryopreservation and in vitro culture Theriogenology, 97, 139-147 (2017)

PubMed: 28583597

Reactivity

Equine

Blank control (blue line): Hela (fixed with 80% methanol (5 min at -20°C) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature). Primary Antibody (green line): Rabbit Anti-Bax antibody (orb312174), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, dilution: 1 µg/Test.

Sample: Hela (Human) Cell Lysate at 30 ug, Primary: Anti-Bax (orb312174) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 21 kD, Observed band size: 23 kD.