BAX Rabbit Polyclonal Antibody

SKU: orb213599

Description

The BAX Antibody is suitable for ELISA, IF, IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the center region of human BAX. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

Research Area

Apoptotic, Cancer, Epigenetics, Infectious Diseases, Neuroscience, Signaling Pathways

Images & Validation

• Tested Applications: ELISA, IF, IHC, WB
• Dilution Range: ELISA: 1-5000-1-10000, WB: 1-500-1-1000, IHC-P: 1-100-1-200, IF/ICC: 1-100-1-500
• Reactivity: Bovine, Human, Mouse, Porcine, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human BAX. The exact sequence is proprietary.
• Target: BAX
• Molecular Weight: 21 kDa
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

BCL2L4; Apoptosis regulator BAX; Bcl-2-like protein 4; Bcl2-L-4

Immunofluorescent analysis of BAX staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Immunohistochemical analysis of paraffin-embedded rat kidney using BAX Antibody orb213599 with dilution at 1:100.

Immunohistochemical analysis of paraffin-embedded human breast cancer using BAX Antibody orb213599 with dilution at 1:100.

Western blot analysis of mouse brain (1), rat lung (2) using BAX Antibody orb213599 with dilution at 1:1000.

Quick Database Links

Gene Symbol

BAX

UniProt

Q63690, Q07813, Q07812

NCBI Gene ID

581, 12028