Bak rabbit pAb

SKU: orb764621

Description

Rabbit polyclonal antibody to Bak

Images & Validation

• Tested Applications: ELISA, IF, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. IF 1:100-300 Not yet tested in other applications.
• Reactivity: Human, Mouse

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human Bak. AA range:1-50
• Molecular Weight: 25
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-BAK1 antibody, anti-BAK antibody, anti-BCL2L7 antibody, anti-CDN1 antibody, anti-Bcl-2 homologous antagonist/killer antibody, anti-Apoptosis regulator BAK antibody, anti-Bcl-2-like protein 7 antibody, anti-Bcl2-L-7 antibody

Immunofluorescence analysis of A549. 1, primary Antibody was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 488 Secondary antibody was diluted at 1:1000 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min.

Immunofluorescence analysis of Hela cell. 1, Bak Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). (red) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min).

Western Blot analysis of various cells using Bak Polyclonal Antibody diluted at 1:500.

Western Blot analysis of 293 cells using Bak Polyclonal Antibody diluted at 1:500.