Bak Antibody (BH3 Domain Specific)

• Catalog Number: orb1937002
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB04682
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Dilution range: WB: 1:2000, IHC-P: 1:50~100
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 23409 Da
• Target: This Bak antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 56-91 amino acids from human Bak.
• UniProt ID: Q16611
• NCBI: NP_001179.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Bcl-2 homologous antagonist/killer, Apoptosis regu
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-hBak-BH3 at 1:2000 dilution. Lane 1: THP-1 whole cell lysate. Lane 2: Jurkat whole cell lysate. Lane 3: MDA-MB-453 whole cell lysate. Lane 4: 293 whole cell lysate. Lane 5: RPMI 8226 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 23 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.