BAG3 Antibody

• Catalog Number: orb1259772
• Category: Antibodies
• Description: BAG3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 380-575 of human BAG3 (NP_004272.2).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100IP: 1:50 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 80kDa
• Target: BAG3
• UniProt ID: O95817
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: BAG3, BCL2-associated athanogene 3 , BAG-3, BIS, C
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100IP: 1:50 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using BAG3 antibody (orb1259772) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunohistochemistry of paraffin-embedded rat heart using BAG3 antibody (orb1259772) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human lung using BAG3 antibody (orb1259772) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human lung cancer using BAG3 antibody (orb1259772) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human breast cancer using BAG3 antibody (orb1259772) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse heart using BAG3 antibody (orb1259772) at dilution of 1:100 (40x lens).