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Catalog Number | orb18594 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to BAG3 |
Target | BAG3 / BIS/ CAIR1 |
Clonality | Polyclonal |
Species/Host | Goat |
Conjugation | Unconjugated |
Reactivity | Human |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Protein Sequence | SSMTDTPGNPAAP |
RRID | AB_10748812 |
MW | 61.6 |
Tested applications | ELISA, IHC, WB |
Dilution range | ELISA: 1:2000, WB: 0.3-1 μg/ml, IHC-P: 3.75 μg/ml |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 85kDa band observed in Human Skeletal Muscle lysates (calculated MW of 61.6kDa according to NP_004272.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Iwasaki et al, Cancer Res. 2007 Nov 1;67(21):10252-9. PMID: 17974966). Recommended concentration: 0.1-0.3 μg/ml. |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | Anti-BAG 3 antibody, anti-BAG family molecular cha Read more... |
Note | For research use only |
Entrez | 9531 |
Western blot analysis of Human Skeletal Muscle lysate using BAG3 antibody
Immunohistochemical staining of Human Heart using BAG3 antibody
Immunohistochemical staining of Human Skeletal Muscle using BAG3 antibody
Immunohistochemical staining of Human Placenta using BAG3 antibody