B3GALNT1 Antibody

SKU: orb1256204

Description

B3GALNT1 Antibody

Images & Validation

• Tested Applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IF: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 44-331 of human B3GALNT1 (NP_003772.1).
• Target: B3GALNT1
• Molecular Weight: Observed: 45kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

B3GALT3, GLCT3, GLOB, Gb4Cer, P, P1, beta3Gal-T3, galT3, UDP-GalNAc:beta-1,3-N-acetylgalactosaminyltransferase 1, P antigen synthase, P blood group globoside, UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 3 (Globoside blood group), UDP-GalNAc:betaGlcNAc beta-1,3-galactosaminyltransferase, polypeptide 1 (Globoside blood group), UDP-N-acetylgalactosamine:globotriaosylceramide beta-1,3-N-acetylgalactosaminyltransferase, b3Gal-T3, beta-1,3-GalNAc-T1, beta-1,3-GalTase 3, beta-1,3-galactosyltransferase 3, beta-3-Gx-T3, brainiac1, galactosylgalactosylglucosylceramide beta-D-acetyl-galactosaminyltransferase, globoside synthase, globotriaosylceramide 3-beta-N-acetylgalactosaminyltransferase

Western blot analysis of extracts of various cell lines, using B3GALNT1 antibody (orb1256204) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Immunofluorescence analysis of U2OS cells using B3GALNT1 antibody (orb1256204) at dilution of 1:100. Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

B3GALNT1

UniProt

O75752