ATP5B Antibody

• Catalog Number: orb1257339
• Category: Antibodies
• Description: ATP5B Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 230-529 of human ATP5B (NP_001677.2).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 57kDa
• Target: ATP5B
• UniProt ID: P06576
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: ATP5B, ATPMB, ATPSB, MGC5231, HEL-S-271
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using ATP5B Antibody (orb1257339) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Immunohistochemistry of paraffin-embedded rat heart using ATP5B antibody (orb1257339) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human lung cancer using ATP5B antibody (orb1257339) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human gastric cancer using ATP5B antibody (orb1257339) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse heart using ATP5B antibody (orb1257339) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using ATP5B antibody (orb1257339) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using ATP5B antibody (orb1257339) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC-12 cells using ATP5B antibody (orb1257339) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.