ATG5/APG5L Mouse Monoclonal Antibody

• Catalog Number: orb1602288
• Category: Antibodies
• Description: ATG5/APG5L Mouse Monoclonal Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 10C4
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant human ATG5 Protein
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-1000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 32 kDa
• Target: ATG5
• UniProt ID: Q9H1Y0
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: ATG5; APG 5; APG 5L; APG5; APG5 autophagy 5 like;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (mouse kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (ATG5/APG5L) Polyclonal Antibody, Unconjugated (orb1602288) at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-mouse IgG antibody for 90 minutes, and DAPI for nuclei staining.

Paraformaldehyde-fixed, paraffin embedded (rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (ATG5/APG5L) Polyclonal Antibody, Unconjugated (orb1602288) at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-mouse IgG antibody for 90 minutes, and DAPI for nuclei staining.

Sample: Lane 1: Human PANC-1 cell lysates, Lane 2: Human MCF-7 cell lysates, Primary: Anti-ATG5/APG5L (orb1602288) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 32 kDa, Observed band size: 47 kDa.