ATG4D Antibody

• Catalog Number: orb1939373
• Category: Antibodies
• Description: ATG4D Antibody
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG1,K
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• UniProt ID: Q86TL0
• MW: 52922 Da
• Tested applications: IF, WB
• Dilution range: WB - 1:4000
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: APG4D, AUTL4
• Research Area: Cancer Biology, Cardiovascular Research, Cell Biol
• Note: For research use only

Anti-ATG4D at 1:4000 dilution + A431 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 53 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunofluorescent analysis of U251 cells, using ATG4D Antibody. U251 cells (right) were treated with Chloroquine (50 μM, 16h). diluted at 1:25 dilution. Dylight Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).