ATG4B Antibody

• Catalog Number: orb248171
• Category: Antibodies
• Description: Autophagy is the cellular mechanism of degradation and turnover of unnecessary or dysfunctional cellular components. The unneeded components are engulfed in vesicles called autophagosomes, where they are broken down and recycled. The ‘autophagy-related gene’ ATG4B is a regulator of autophagasome maturation. The microtubule-associated protein LC3-I is lipidated (called LC3-II in its lipidated state) and recruited to the autophagosomal membrane where it is of vital importance in the maturation of autophagosomes. ATG4B regulates LC3-II availability by modulating LC3-I lipidation.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Immunogen: A recombinant protein fragment from the C-terminal region of ATG4B was used as the immunogen for this antibody.
• Dilution range: Western blot: 4-8ug/ml
• Purity: Protein A affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.5 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This ATG4B antibody is available for research use only.
• Entrez: 23192
• Storage: Aliquot the ATG4B antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: 1X PBS, pH 7.4
• Note: For research use only
• Application notes: Provided assay concentrations are suggestions only, ATG4B antibody titration may be required for optimal results.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of human samples using ATG4B antibody (primary antibody dilution at: at 4 ug/ml)