ATG4B Antibody (N-term)

• Catalog Number: orb1933482
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB7496
• Tested applications: IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, WB: 1:1000, WB: 1:1000, IHC-P: 1:50~100
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 44294 Da
• Target: This ATG4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 16-45 amino acids from the N-terminal region of human ATG4B.
• UniProt ID: Q9Y4P1
• NCBI: NP_847896.1, NP_037457.3
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Cysteine protease ATG4B, 3422-, AUT-like 1 cystein
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

The anti-APG4B Pab is used in Western blot to detect APG4B in CEM tissue lysate

APG4B Antibody (R31) western blot analysis in mouse cerebellum tissue lysates (35 ug/lane). This demonstrates the APG4B antibody detected the APG4B protein (arrow).

Western blot analysis of anti-hAPG4B-R31 Pab in 293 cell line lysates transiently transfected with the ATG4B gene (2 ug/lane). hAPG4B-R31 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.