PPP1R13B Antibody

SKU: orb345587

Description

ASPP1 antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:2,500 - 1:10,000, WB: 1:500 - 1:3,000
• Reactivity: Human
• Application Notes:
  This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 120 kDa in size corresponding to ASPP1 by western blotting in the appropriate cell lysate or extract.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal sequence of human ASPP1.
• Target: PPP1R13B
• Purity: This affinity purified antibody is directed against human ASPP1. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. Minimal reactivity occurs against ASPP2. A BLAST analysis was used to suggest cross-reactivity with ASPP1 from mouse based on a 92% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 0.33 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-ASPP1 Antibody, ASPP-1, ASPP 1, p53BP2 like antibody, p85 antibody, PPP1R13B antibody

Western blot using Biorbyt's affinity purified anti-ASPP1 to detect over-expressed ASPP1 in MCF-7 cells (lane 2, arrowhead). Lane 1 is a non-transfected control. Lane 3 is MCF-7 cells over-expressing ASPP2. Cell extracts were electrophoresed and transferred to nitrocellulose. The membrane was probed with the primary antibody at a 1:1000 dilution. The identity of the lower MW band at approximately 50kDa is unknown. Primary experimental data indicate that the unknown band intensifies in extracts from p53 siRNA knockdown cells.

Quick Database Links

Gene Symbol

PPP1R13B

UniProt

Q96KQ4

RefSeq (Protein):NP_056131.2