MAP3K5 Antibody

SKU: orb1240198

Description

MAP3K5 Antibody

Research Area

Cell Biology, Signal Transduction

Images & Validation

• Tested Applications: ELISA, ICC, IF, WB
• Reactivity: Human
• Predicted Reactivity: Mouse
• Application Notes:
  ASK1 antibody can be used for detection of ASK1 by Western blot at 0.5 μg/mL. A 155 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: ASK1 antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human ASK1.The sequence is different from that of mouse by last two amino acids.The immunogen is located within the last 50 amino acids of ASK1.
• Target: MAP3K5
• Molecular Weight: 155 kDa
• Purification: ASK1 Antibody is DEAE purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: ASK1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ASK1 Antibody: ASK1, MEKK5, MAPKKK5, ASK1, Mitogen-activated protein kinase kinase kinase 5, Apoptosis signal-regulating kinase 1, ASK-1

Western blot analysis of ASK1 in SW1353 whole cell lysate with ASK1 antibody at 1:500 dilution.

Immunocytochemistry of ASK1 in A431 cells with ASK1 antibody at 10 µg/mL.

Immunofluorescence of ASK1 in A431 cells with ASK1 antibody at 20 µg/mL.

Quick Database Links

Gene Symbol

MAP3K5

UniProt

Q99683

RefSeq:Q99683