MAP3K5 Antibody

SKU: orb1240197

Description

MAP3K5 Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, ICC, IF, WB
• Reactivity: Human, Mouse
• Application Notes:
  ASK1 antibody can be used for the detection of ASK1 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: ASK1 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human ASK1.The immunogen is located within amino acids 1260 - 1310 of ASK1.
• Target: MAP3K5
• Purification: ASK1 Antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: ASK1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ASK1 Antibody: ASK1, MEKK5, MAPKKK5, ASK1, Mitogen-activated protein kinase kinase kinase 5, Apoptosis signal-regulating kinase 1, ASK-1

Western blot analysis of ASK1 in SW1353 cell lysate with ASK1 antibody at (A) 0.5, (B) 1, and (C) 2 µg/mL.

Immunocytochemistry of ASK1 in A431 cells with ASK1 antibody at 2 µg/mL.

Immunofluorescence of ASK1 in 3T3 cells with ASK1 antibody at 20 µg/mL.

Quick Database Links

Gene Symbol

MAP3K5

UniProt

Q99683

RefSeq:Q99683