ASAP1 antibody

• Catalog Number: orb345530
• Category: Antibodies
• Description: ASAP1 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, WB
• Reactivity: Mouse
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 775-800 of mouse ASAP1 protein.
• Concentration: 0.95 mg/mL
• Dilution range: ELISA: 1:2,000 - 1:10,000, IF: User Optimized, WB: 1:500 - 1:2,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This affinity-purified antibody is directed against mouse ASAP1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Reactivity occurs against both the phosphorylated and non-phosphorylated forms of the protein at residue Y782. A BLAST analysis was used to suggest cross reactivity with ASAP1 proteins from human, chicken, bovine, dog, rat and chimpanzee based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
• Conjugation: Unconjugated
• UniProt ID: Q9QWY8
• NCBI: 65301464
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-ASAP1 Antibody, ASAP-1, ASAP 1, Develo
• Note: For research use only
• Application notes: This affinity purified antibody has been tested for use in ELISA, IF microscopy and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 130 kDa in size corresponding to ASAP1 protein by western blotting in the appropriate cell lysate or extract. This antibody recognizes both phosphorylated and non-phosphorylated ASAP1 at amino acid Y782.
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of mouse NIH3T3 cells using ASAP1 antibody