ASAP1 (phospho-Y782) antibody

• Catalog Number: orb345529
• Category: Antibodies
• Description: ASAP1 (phospho-Y782) antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 775-800 of mouse ASAP1 protein.
• Concentration: 1.00 mg/mL
• Dilution range: ELISA: 1:4,000 - 1:16,000, IHC: 20-40 µg/ml, WB: 1:500 - 1:2,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This affinity-purified antibody is directed against the phosphorylated form of mouse ASAP1 protein at the pY782 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against mouse ASAP1 pY782 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated mouse ASAP1 is minimal by ELISA. A BLAST analysis was used to suggest cross reactivity with ASAP1 proteins from human, chicken, bovine, dog, rat and chimpanzee based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
• Conjugation: Unconjugated
• UniProt ID: Q9QWY8
• NCBI: 65301464
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-ASAP1 pY782 Antibody, ASAP-1, ASAP 1,
• Note: For research use only
• Application notes: This affinity purified antibody has been tested for use in ELISA, immunohistochemistry, IF microscopy and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 130 kDa in size corresponding to phosphorylated ASAP1 protein by western blotting in the appropriate cell lysate or extract. Less than 2.5% reactivity is observed against the non-phosphorylated form of the immunizing peptide. This antibody is phospho specific for pY782 of ASAP1 protein.
• Expiration Date: 12 months from date of receipt.

References

Ruggiero, Carmen et al. The KDEL receptor signalling cascade targets focal adhesion kinase on focal adhesions and invadopodia Oncotarget, 9, 10228-10246 (2018)

PubMed: 29535802

Immunofluorescence analysis of mouse NIH3T3 cells transfected lysates using ASAP1 (phospho-Y782) antibody

Immunohistochemical staining of variety of tissues including multi-human, multi-brain and multi-cancer slides using ASAP1 (phospho-Y782) antibody