ASS1 Antibody

SKU: orb1246755

Description

ASS1 Antibody

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 45kDa band observed in lysates of cell lines A431 and NIH3T3 and approx.48kDa in Human and Rat Kidney and in Mouse Liver lysates (calculated MW of 46.5kDa according to Human NP_000041.2 and Rat NP_037289.1, and 46.6kDa according to Mouse NP_031520.1 ). Recommended concentration: 0.03-1ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:In paraffin embedded Human Kidney shows textured cytoplasm staining in PCT. Recommended concentration: 2-4ug/ml. Paraffin embedded Human Liver. Recommended concentration: 2.5ug/ml.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-ENPKNQAPPGLYTKTQD
• Target: ASS1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Disclaimer: For research use only

Alternative Names

Argininosuccinate synthetase 1, ASS, ASS1, CTLN1, citrulline--aspartate ligase

orb1246755 (2.5 ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1246755 (2.5 ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1246755 (0.3 ug/ml) staining of A431 (A) and (1 ug/ml) NIH3T3 (B) cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1246755 (0.01 ug/ml) staining of Human Kidney (A) Mouse Liver (B) and (0.03 ug/ml) Rat Kidney (C) lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1246755 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI.

Quick Database Links

Gene Symbol

ASS1

UniProt

P00966

RefSeq (Protein):NP_446464.1, NP_000041.2