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Arginase 1 Antibody

Catalog Number: orb749618

DispatchUsually dispatched within 5-10 working days
$ 410.00
Catalog Numberorb749618
CategoryAntibodies
DescriptionRecognizes a protein of 35-38kDa, which is identified as Arginase 1 (ARG1). Arginase is a manganese metallo-enzyme that catalyzes the hydrolysis of arginine to generate ornithine and urea. Arginase I and II are isoenzymes which differ in subcellular localization, regulation, and possibly function. Arginase I is a cytosolic enzyme, which is expressed mainly in the liver as part of the urea cycle, whereas arginase II is a mitochondrial protein found in a variety of tissues. Antibody to ARG-1 labels hepatocytes in normal tissues and granulocytes in peripheral blood. ARG-1 is a sensitive and specific marker for identification of hepatocellular carcinoma.
Species/HostMouse
ClonalityMonoclonal
Clone NumberARG1/1125
Tested applicationsIHC-P, WB
ReactivityHuman
IsotypeMouse IgG3, kappa
ImmunogenA recombinant fragment from amino acids 1-150 of human ARG1 was used as the immunogen for the Arginase 1 antibody.
Antibody TypePrimary Antibody
Dilution rangeWestern blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-4ug/ml for 30 min at RT
PurityProtein G affinity chromatography
ConjugationUnconjugated
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Hazard InformationThis Arginase 1 antibody is available for research use only.
UniProt IDP05089
StorageStore the Arginase 1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Buffer/Preservatives0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
NoteFor research use only
Application notesOptimal dilution of the Arginase 1 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Expiration Date12 months from date of receipt.
Arginase 1 Antibody

Western blot analysis of A) partial recombinant ARG1 protein and B) human liver lysate using Arginase 1 antibody (ARG1/1125). Predicted molecular weight ~35 kDa.

Arginase 1 Antibody

IHC: Formalin-fixed, paraffin-embedded human hepatocellular carcinoma stained with Arginase 1 antibody (ARG1/1125). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT prior to testing.

Arginase 1 Antibody

Analysis of HuProt (TM) microarray containing more than 19, 000 full-length human proteins using Arginase 1 antibody (clone ARG1/1125). These results demonstrate the foremost specificity of the ARG1/1125 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Arginase 1 Antibody

SDS-PAGE analysis of purified, BSA-free Arginase 1 antibody (clone ARG1/1125) as confirmation of integrity and purity.

Arginase 1 Antibody

Western blot analysis of human liver lysate using Arginase 1 antibody (ARG1/1125). Predicted molecular weight ~35 kDa.

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