ARG1 Antibody

• Catalog Number: orb1249601
• Category: Antibodies
• Description: ARG1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine, Canine
• Reactivity: Human, Mouse, Porcine, Rat
• Immunogen: The immunogen for this antibody is: CFGLAREGNHKPID
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx. 37kDa band observed in Human, Mouse, Rat and Pig Liver lysates (calculated MW of 34.7kDa according to NP_000036.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.01-0.03ug/ml. Primary incubation was 1 hour.Immunohistochemistry:In paraffin embedded Human Kidney shows preferential staining of PCT. Recommended concentration: 2-4ug/ml. Paraffin embedded Human Liver. Recommended concentration: 3.75ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: ARG1
• UniProt ID: P05089
• NCBI: NP_000036.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: ARG1, arginase, liver , HGNC:663 , A-I, arginase,
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx. 37kDa band observed in Human, Mouse, Rat and Pig Liver lysates (calculated MW of 34.7kDa according to NP_000036.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.01-0.03ug/ml. Primary incubation was 1 hour.Immunohistochemistry:In paraffin embedded Human Kidney shows preferential staining of PCT. Recommended concentration: 2-4ug/ml. Paraffin embedded Human Liver. Recommended concentration: 3.75ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1249601 (0.01 ug/ml) staining of Human Liver lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1249601 (0.03 ug/ml) staining of Pig (A), Mouse (B) and Rat (C) Liver lysates (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1249601 (4 ug/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

orb1249601 (3.75 ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.