ARG1 Antibody

SKU: orb1249601

Description

ARG1 Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human, Mouse, Porcine, Rat
• Predicted Reactivity: Bovine, Canine
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx. 37kDa band observed in Human, Mouse, Rat and Pig Liver lysates (calculated MW of 34.7kDa according to NP_000036.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.01-0.03µg/mL. Primary incubation was 1 hour.Immunohistochemistry:In paraffin embedded Human Kidney shows preferential staining of PCT. Recommended concentration: 2-4µg/mL. Paraffin embedded Human Liver. Recommended concentration: 3.75µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: CFGLAREGNHKPID
• Target: ARG1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ARG1, arginase, liver , HGNC:663 , A-I, arginase, type I, arginase 1, liver-type arginase, type I arginase

orb1249601 (0.01 ug/ml) staining of Human Liver lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1249601 (0.03 ug/ml) staining of Pig (A), Mouse (B) and Rat (C) Liver lysates (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1249601 (4 ug/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

orb1249601 (3.75 ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

ARG1

UniProt

P05089

RefSeq (Protein):NP_000036.2