ARFGEF1 Antibody

SKU: orb1258986

Description

ARFGEF1 Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: WB
• Reactivity: Human
• Application Notes:
  WB: 1:500 - 1:2000

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 220-420 of human ARFGEF1 (NP_006412.2).
• Target: ARFGEF1
• Molecular Weight: Observed: 200kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ARFGEP1, BIG1, P20brefeldin A-inhibited guanine nucleotide-exchange protein 1, ADP-ribosylation factor guanine nucleotide-exchange factor 1 (brefeldin A-inhibited), p200 ARF guanine nucleotide exchange factor

Western blot analysis of extracts of various cell lines, using ARFGEF1 antibody (orb1258986) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Quick Database Links

Gene Symbol

ARFGEF1

UniProt

Q9Y6D6