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Catalog Number | orb1529248 |
---|---|
Category | Antibodies |
Description | BIG1/ARFGEF1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, IP, WB |
Reactivity | Human, Mouse |
Immunogen | Between 1799 and C-terminus |
Antibody Type | Primary Antibody |
Concentration | 1000 µg/ml |
Dilution range | WB - 1:1,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:1,000 to 1:5,000. Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections. |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | BIG1/ARFGEF1 |
UniProt ID | Q9Y6D6 |
NCBI | NP_006412.2 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
Alternative names | brefeldin A-inhibited guanine nucleotide-exchange Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human BIG1/ARFGEF1 by western blot.Samples: Whole cell lysate from RKO, LNCaP, HEK293T, Jurkat, and GaMG cells. Antibody: Affinity purified rabbit anti-BIG1/ARFGEF1 antibody at 0.1 µg/ml.
Detection of human BIG1/ARFGEF1 by western blot.Samples: Whole cell lysate from NIH 3T3, TCMK-1, and CT26 cells. Antibody: Affinity purified rabbit anti-BIG1/ARFGEF1 antibody at 0.1 µg/ml.
Detection of human BIG1/ARFGEF1 by western blot of immunoprecipitates.Samples: Whole cell lysate from RKO cells. Antibodies: Affinity purified rabbit anti-BIG1/ARFGEF1 antibody.
IHC, IP, WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |