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Catalog Number | orb1260792 |
---|---|
Category | Antibodies |
Description | AREG Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 20-252 of human AREG (NP_001648.1). |
Concentration | batch dependent |
Dilution range | WB: 1:1000 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 44kDa |
Target | AREG |
UniProt ID | P15514 |
Storage | Store at -20°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
Alternative names | Amphiregulin, AR, Colorectum cell-derived growth f Read more... |
Note | For research use only |
Application notes | WB: 1:1000 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using AREG antibody (orb1260792) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human liver using AREG antibody (orb1260792) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human tonsil using AREG antibody (orb1260792) at dilution of 1:100 (40x lens).
IHC-P, WB | |
Bovine, Canine, Equine, Rabbit, Sheep |
ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Polyclonal | |
Unconjugated |
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