Aquaporin 2 Antibody: HRP

• Catalog Number: orb152804
• Category: Antibodies
• Description: Rabbit polyclonal antibody against Aquaporin 2 Conjugated to HRP
• Target: Aquaporin 2
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: HRP
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: 73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide and 0.23mM Citrate in dH2O
• Purification: Protein A Purified
• Immunogen: Produced against the C-terminal peptide (Sequence N-CLKGLEPDTDWEEREVRRRQ) of rat aquaporin 2
• UniProt ID: P34080
• MW: 35-50kDa
• Tested applications: ELISA, IHC, WB
• Dilution range: WB (1:2000), IHC (1:200), ICC/IF (1:400)
• Application notes: 0.5 µg/ml of SPC-503 was sufficient for detection of aquaporin 2 in 10 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
• Storage: Conjugated antibodies should be stored according to the product label
• Alternative names: ADH water channel antibody, AQP 2 antibody, AQP CD
• Note: For research use only
• Entrez: 25386
• NCBI: NP_037041.2

Immunohistochemistry analysis using Rabbit Anti-Aquaporin 2 Polyclonal Antibody. Tissue: kidney tissue. Species: Rat. Fixation: 4% paraformaldehyde-lysine-periodate. Primary Antibody: Rabbit Anti-Aquaporin 2 Polyclonal Antibody at 1:200 for 75 min at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) for 1 hour at RT. 4 μm cryostat sections.

Western blot analysis of Rat kidney inner medullary homogenates showing detection of Aquaporin 2 protein using Rabbit Anti-Aquaporin 2 Polyclonal Antibody. Primary Antibody: Rabbit Anti-Aquaporin 2 Polyclonal Antibody at 1:2000. Showing glycosylated and non-glycosylated bands.