Aquaporin 4 Antibody (HRP)

SKU: orb152838

Description

Rabbit polyclonal antibody against Aquaporin 4 Conjugated to HRP

Images & Validation

• Tested Applications: ICC, IF, IHC, WB
• Dilution range: WB (1:2000), IHC (1:200), ICC/IF (1:400)
• Reactivity: Human, Mouse, Rat
• Application Notes:
  0.5 µg/ml of SPC-505 was sufficient for detection of aquaporin 4 in 10 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Produced against the C-terminal peptide (Sequence N-CTKGSYMEVEDNRSQVETED) of rat aquaporin 4
• Target: Aquaporin 4
• Molecular Weight: 50kDa
• Purification: Protein A Purified
• Conjugation: HRP

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide, 0.23mM Citrate
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Aquaporin 4, Aquaporin-4, AQP4, AQP-4, AQP 4, AQP4_HUMAN, Aquaporin type 4, Aquaporin4, HMIWC 2, HMIWC2, Mercurial insensitive water channel, Mercurial-insensitive water channel, MGC22454, MIWC, WCH 4, WCH4

Immunohistochemistry analysis using Rabbit Anti-Aquaporin 4 Polyclonal Antibody. Tissue: kidney tissue. Species: Rat. Fixation: 4% paraformaldehyde-lysine-periodate. Primary Antibody: Rabbit Anti-Aquaporin 4 Polyclonal Antibody at 1:200 for 75 min at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) for 1 hour at RT. 4 μm cryostat sections.

Western blot analysis of Rat kidney inner medullary homogenates showing detection of Aquaporin 4 protein using Rabbit Anti-Aquaporin 4 Polyclonal Antibody. Primary Antibody: Rabbit Anti-Aquaporin 4 Polyclonal Antibody at 1:2000.

Quick Database Links

Gene Symbol

Aquaporin 4

UniProt

P47863

NCBI Gene ID

25293

RefSeq (Protein):NP_036957.1