APOM Antibody

SKU: orb1247784

Description

APOM Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, IHC
• Reactivity: Human
• Predicted Reactivity: Canine
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Preliminary experiments gave an approx 50kDa band in Human Kidney and Liver lysates after 0.2µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 21.3kDa according to NP_061974.2. We do note that ApoM is a glycoprotein which makes a higher size band to be expected. The 50kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Liver shows strong stain of cytoplasm in select hepatocytes. Recommended concentration, 3-6µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: PRNQEACELSNN
• Target: APOM
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

APOM, apolipoprotein M , G3a, HSPC336, MGC22400, NG20 , NG20-like protein, G3a, NG20

orb1247784 (5 ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

APOM

UniProt

O95445

RefSeq (Protein):NP_061974.2