APOM Antibody

• Catalog Number: orb19417
• Category: Antibodies
• Description: Goat polyclonal antibody to APOM
• Target: APOM
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Canine
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: PRNQEACELSNN
• RRID: AB_10762497
• MW: 21.3
• Tested applications: ELISA, IHC
• Dilution range: ELISA: 1:8000, WB: 0.2 μg/ml, IHC-P: 3-6µg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.IHC: In paraffin embedded Human Liver shows strong stain of cytoplasm in select hepatocytes. Recommended concentration, 3-6ug/ml.WB: Preliminary experiments gave an approx 50kDa band in Human Kidney and Liver lysates after 0.2 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 21.3kDa according to NP_061974.2. We do note that ApoM is a glycoprotein which makes a higher size band to be expected. The 50kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti APOM antibody, anti apolipoprotein M antibody
• Research Area: Epigenetics
• Note: For research use only
• Entrez: 55937

orb19417 (5ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.