APOBEC3D Antibody

SKU: orb1248857

Description

APOBEC3D Antibody

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 45kDa band observed in lysates of cell line A549 (calculated MW of 46.6kDa according to NP_689639.2). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Spleen. Recommended concentration: 3.75ug/ml.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-PKRQSNHRQEVYFR
• Target: APOBEC3D
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Disclaimer: For research use only

Alternative Names

APOBEC3D, apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3D, APOBEC3DE, APOBEC3E, ARP6, DNA dC->dU-editing enzyme APOBEC-3D, apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3E pseudogene, probable DNA dC->dU-editing enzyme APOBEC-3D

orb1248857 (0.3 ug/ml) staining of A549 lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248857 (3.75 ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

APOBEC3D

UniProt

Q96AK3

RefSeq (Protein):NP_689639.2