TFAP2C Antibody

SKU: orb1247793

Description

TFAP2C Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, IHC
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:WB experiments gave bands at approx 75kDa and 22kDa in Human Placenta and Ovary lysates after 0.1µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 49.2kDa according to NP_003213.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Skin shows nuclear staining of keratinocytes. Recommended concentration, 2-3µg/mL.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-NKTLEKMEKHRK
• Target: TFAP2C
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

TFAP2C, ERF1, TFAP2G, hAP-2g, AP2-GAMMA, transcription factor AP-2 gamma (activating enhancer binding protein 2 gamma), estrogen receptor factor 1, transcription factor AP-2 gamma (activating enhancer-binding protein 2 gamma)

Quick Database Links

Gene Symbol

TFAP2C

UniProt

Q92754

RefSeq (Protein):NP_003213.1