Anticancer agent 43

SKU: orb2900832

Description

Anticancer Agent 43 is a potent anticancer agent. Anticancer Agent 43 induces apoptosis by caspase 3, PARP1, and Bax dependent mechanisms. Anticancer Agent 43 induces DNA damage.

Key Properties

• CAS Number: 2470015-35-9
• MW: 336.36
• Purity: >98% (HPLC)
• Formula: C₁₄H₉FN₂O₃S₂
• SMILES: C(C=1C=2C(NC1C(OC)=O)=CC=C(F)C2)=C3C(=O)NC(=S)S3
• Target: Apoptosis
• Solubility: In Vitro: DMSO : 125 mg/mL (371.63 mM; Ultrasonic )

Bioactivity

• In Vitro:
  Anticancer agent 43 (compound 3a) shows selectivity toward human tumor cells (SI50=28.94). Anticancer agent 43 (45 μM, 24 h) induces apoptosis via caspase 3, PARP1 and Bax dependent pathways in HepG2 cells. Anticancer agent 43 (45 μM, 24 h) shows no effect on the transition of G1/S phases in HepG2 cells. Anticancer agent 43 (0.7, 45, 55 μM) induces DNA damage in HCT116 cells (Tail DNA=16.1%, OTM=3.7), MCF-7 cells, HepG2 cells (Tail DNA=26.2%, OTM=13.2), Balb/c 3T3 cells (Tail DNA = 8.4%, OTM = 3.5).. Cell Cytotoxicity Assay Cell line: HepG2, MCF-7, HCT116, HeLa, A549, WM793, THP-1, HaCaT, Balb/c3T3 cells. Concentration: 0, 1, 10, 100 μM. Incubation time: 72 h. Result: Showed cytotoxic action with GI50s of 12.1, 0.7, 0.8, 49.3, 9.7 μM for for HepG2, MCF-7, HCT116, HeLa, A549 cells, low toxicity towards WM793, THP-1, HaCaT, Balb/c 3T3 cells with GI50s of 80.4, 62.4, 98.3, 40.8 μM, respectively. Apoptosis Analysis Cell line: HepG2 cells. Concentration: 45 μM. Incubation time: 24 h. Result: Induced apoptosis in HepG2 cells via caspase 3, PARP1 and Bax dependent pathways. Western blot analysis. Cell line: HCT116, MCF-7 cells. Concentration: 0.7 μM Incubation time: 24 h. Result: Decreased the expression of Cdk2 protein in HCT116 and MCF-7 cells. Cell Cycle Analysis Cell line: HepG2 cells. Concentration: 45 μM. Incubation time: 24 h. Result: Showed no effect on the transition of G1/S phases in HepG2 cells.
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Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

Apoptosis