WNK1 (Phospho-T60) Antibody

• Catalog Number: orb304662
• Category: Antibodies
• Description: Rabbit polyclonal antibody to p65 (phospho-T60)
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Porcine, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T60 of human WNK1 protein. The exact sequence is proprietary.
• UniProt ID: P83741, Q9H4A3, Q9JIH7
• Tested applications: IHC, WB
• Dilution range: WB: 1:500-1000, IHC-P: 1:100-200
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: HSN2; KDP; KIAA0344; PRKWNK1; Serine/threonine-pro
• Research Area: Epigenetics, Neuroscience
• Note: For research use only
• Entrez: 116477, 232341, 65125
• Expiration Date: 12 months from date of receipt.

References

Feng, Shandan et al. Resveratrol Plays Protective Roles on Kidney of Uremic Rats via Activating HSP70 Expression Biomed Res Int, 2020, 2126748 (2020)

PubMed: 32280682

Applications

WB

Reactivity

Rat

Yi, Lanjie et al. Formononetin inhibits inflammation and promotes gastric mucosal angiogenesis in gastric ulcer rats through regulating NF-κB signaling pathway J Recept Signal Transduct Res, (2020)

PubMed: 33100111

Applications

WB

Reactivity

Rat

Western blot analysis of WNK1 (Phospho-T60) expression in HEK293T EGF-treated (A), Jurkat EGF-treated (B) whole cell lysates. (Predicted band size: 250 kD; Observed band size: 230 kD)

Immunohistochemical analysis of WNK1 (Phospho-T60) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.