Phospho-WNK1 (Thr60) Rabbit Polyclonal Antibody

• Catalog Number: orb1974704
• Category: Antibodies
• Description: Phospho-WNK1 (Thr60) Rabbit Polyclonal Antibody
• Target: WNK1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Mouse
• Predicted Reactivity: Bovine, Canine, Gallus, Human, Porcine, Rabbit, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human WNK1 around the phosphorylation site of Thr60 RH(p-T)MD
• UniProt ID: Q9H4A3
• MW: 251 kDa
• Tested applications: ELISA, IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: WNK1(phospho Thr60); WNK1(phospho T60); Erythrocyt
• Research Area: Kinases
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (mouse kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (Phospho-WNK1 (Thr60)) Polyclonal Antibody, Unconjugated (orb1974704) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.