Anti-Vimentin Antibody

• Catalog Number: orb323201
• Category: Antibodies
• Description: Mouse monoclonal antibody to Vimentin
• Target: VIM
• Clonality: Monoclonal
• Species/Host: Mouse
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Immunogen: Recombinant protein corresponding to human Vimentin.
• UniProt ID: P08670, P20152, P31000
• Tested applications: IF, IH, WB
• Dilution range: WB: 1:1000-1:3000, IHC-P: 1:200-1:500, IF/ICC: 1:100-1:200
• Antibody Type: Primary Antibody
• Source: Mouse
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti Vimentin antibody
• Note: For research use only
• Entrez: 81818, 7431, 22352

Western blot analysis of Vimentin expression in Hela (A), mouse brain (B), rat brain (C) whole cell lysates. (Predicted band size: 53 kD; Observed band size: 50-57 kD)

Immunohistochemical analysis of Vimentin staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of Vimentin staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).