Anti-UBE2L6 Antibody

• Catalog Number: orb668554
• Category: Antibodies
• Description: Rabbit polyclonal antibody to UBE2L6
• Target: UBE2L6
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Immunogen: KLH-conjugated synthetic peptide of human UBE2L6. The exact sequence is proprietary.
• UniProt ID: Q9QZU9, O14933, Q4V8J2
• Tested applications: IF, WB
• Dilution range: WB: 1-500-2000
• Antibody Type: Primary Antibody
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-UBCH8 antibody, anti-Ubiquitin/ISG15-conjugat
• Note: For research use only
• Entrez: 56791, 295704, 9246

Western blot analysis of UBE2L6 expression in Jurkat (A), mouse lung (B), mouse spleen (C), rat kidney (D), rat liver (E) whole cell lysates. (Predicted band size: 10; 17 kD; Observed band size: 18 kD)

Immunofluorescent analysis of UBE2L6 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).