Anti-UBE2L6 Antibody

• Catalog Number: orb668554
• Category: Antibodies
• Description: Rabbit polyclonal antibody to UBE2L6
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: KLH-conjugated synthetic peptide of human UBE2L6. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Dilution range: WB: 1-500-2000
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: UBE2L6
• Entrez: 56791, 295704, 9246
• UniProt ID: Q9QZU9, O14933, Q4V8J2
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: Anti-UBCH8 antibody, anti-Ubiquitin/ISG15-conjugat
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of UBE2L6 expression in Jurkat (A), mouse lung (B), mouse spleen (C), rat kidney (D), rat liver (E) whole cell lysates. (Predicted band size: 10; 17 kD; Observed band size: 18 kD)

Immunofluorescent analysis of UBE2L6 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).