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Description
Research Area
Images & Validation
−| Tested Applications | ELISA, ICC, IHC, WB |
|---|---|
| Dilution Range | WB: 0.736111111, IHC: 0.736111111, ICC: 0.736111111 |
| Reactivity | Mammal |
| Predicted Reactivity | Gallus |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Sheep |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | SDS-denatured, native rat tyrosine hydroxylase purified from pheochromocytoma. |
| Target | Tyrosine Hydroxylase |
| Molecular Weight | 60 |
| Purification | Antigen Affinity Purified from Pooled Serum |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml rAlbumin and 50% glycerol. |
| Concentration | 0.15mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Immunostaining of free-floating sections of mouse locus ceruleus (brainstem) labeling tyrosine hydroxylase (Cat. orb319701, red, 1:2000) The mouse was transcardially perfused with 20 mL of PBS containing 0.1% procaine and 100 U/mL heparin followed by 20 mL of 4% paraformaldehyde (PFA) in PBS. The dissected brains were kept in fixative at 4 °C overnight.

Immunostaining of rabbit retina showing specific labeling of tyrosine hydroxylase in green.

Western blot of 10ug of rat striatal lysate showing specific immunolabeling of the ~60 kDa tyrosine hydroxylase protein.
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Protocol Information
Tyrosine Hydroxylase Antibody (orb319701)
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