Tyrosine Hydroxylase Antibody

SKU: orb19006

Description

Goat polyclonal antibody to TH

Research Area

Neuroscience

Images & Validation

• Tested Applications: ELISA, IHC
• Dilution Range: ELISA: 1:8000, IHC-P: 1:1000-10000
• Reactivity: Human
• Predicted Reactivity: Canine, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 55kDa band observed in Human Brain (Cerebral Cortex) lysates (calculated MW of 55.5kDa according to NP_000351.2). Recommended concentration: 2-4 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: Tyrosine Hydroxylase
• Protein Sequence: VQDELDTLAHAL
• Molecular Weight: 58.5; 55.5; 58.1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

TH, tyrosine hydroxylase, HGNC:11782, TYH, DYT14, DYT5b, dystonia 14, tyrosine 3-monooxygenase

orb19006 (2ug/ml) staining of Human Cerebral Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemical analysis of staining of Human Cerebral Cortex lysate using TH antibody

Western blot analysis of staining of Human Cerebral Cortex lysate using TH antibody

Immunohistochemical staining of human hypothalamus using TH antibody

Quick Database Links

Gene Symbol

Tyrosine Hydroxylase

NCBI Gene ID

7054