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Description
Research Area
Images & Validation
−| Tested Applications | ELISA, IHC, WB |
|---|---|
| Dilution Range | WB: 1.430555556 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Fusion protein of complete mouse cardiac troponin 1 holoprotein. |
| Target | Troponin I (cardiac) |
| Molecular Weight | 25 |
| Purification | Neat Serum |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Neat serum |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Examination of cardiac troponin I (cTnI) (Cat. orb319691, green) and active β-Catenin (red) levels in embryos at E9.5 by immunofluorescence (IF) analysis. Pregnant mice were treated daily with vehicle or the JAKi (10 mg/kg body weight/day, i.p.) beginning with day 6.5 post-conception. Embryos were harvested at E9.5 for IF analysis.

Western blot of 20 ug of mouse heart lysate showing specific immunolabeling of the ~25 kDa cardiac troponin I protein.

Western blots showing specific labeling of S23/S24 TnI, cat. orb319692 (upper) and TnI; Cat. orb319691 (lower) in protein lysates of mouse cardiomyocytes.
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Protocol Information
Troponin I (cardiac) Antibody (orb319691)
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