TNNI3 (aa117-127) Antibody

• Catalog Number: orb97644
• Category: Antibodies
• Description: Goat polyclonal antibody to TNNI3
• Target: TNNI3 (aa117-127)
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Porcine, Rat
• Predicted Reactivity: Bovine, Canine
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: KVTKNITEIAD
• MW: 24
• Tested applications: ELISA, IF, WB
• Dilution range: ELISA: 1:128000, WB: 0.1-0.3 μg/ml, IHC-P: 10 μg/ml
• Application notes: WB: Approx 28kDa bands observed in Human Heart lysates (calculated MW of 24.0kDa according to NP_000354.4).The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources.Recommended concentration: 0.2-0.5 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti TNNI3 antibody, anti troponin I type 3 (cardi
• Note: For research use only
• Entrez: 7137

Western blot analysis of staining of adult Mouse (A), fetal Mouse (B), adult Rat (C) and adult Pig (D) Heart lysates using Creatine kinase M-type antibody.

Western blot analysis of staining of Human Heart lysate using Creatine kinase M-type antibody.

Immunofluorescence analysis of U2OS cells using TNNI3 antibody

Immunofluorescence analysis of HeLa cells using TNNI3 antibody