TCF4 Rabbit Polyclonal Antibody

SKU: orb340734

Description

The TCF4 Antibody is suitable for IF, WB. It is a Polyclonal, Unconjugated antibody which raised against Recombinant fusion protein of human TCF4 .Purification: The antibody was purified by immunogen affinity chromatography.

Research Area

Developmental Biology, Epigenetics

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: WB: 1:500-1:2000, IF/ICC: 1:50-1:200
• Reactivity: Human, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein of human TCF4
• Target: TCF4
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

BHLHB19; ITF2; SEF2; Transcription factor 4; TCF-4; Class B basic helix-loop-helix protein 19; bHLHb19; Immunoglobulin transcription factor 2; ITF-2; SL3-3 enhancer factor 2; SEF-2

Western blot analysis of TCF4 expression in HepG2 (A), MCF7 (B), mouse brain (C) whole cell lysates. (Predicted band size: 48-71 kD; Observed band size: 71 kD)

Immunofluorescent analysis of TCF4 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Quick Database Links

Gene Symbol

TCF4

UniProt

Q60722, P15884

NCBI Gene ID

6925, 21413