Anti-Tau Oligomeric complex [TOC1]

• Catalog Number: orb1463352
• Category: Antibodies
• Description: Mouse monoclonal antibody to Tau Oligomeric complex
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: TOC1
• Tested applications: ELISA, IF, IHC, WB
• Reactivity: Human
• Isotype: Mouse IgG
• Immunogen: The original antibody was generated by immunizing a mouse with electro eluted cross-linked Tau dimers.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: Tau Oligomeric complex
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: TOC-1;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of Kelly cells with anti-Tau Oligomeric complex TOC1. Immunofluorescence analysis of paraformaldehyde fixed Kelly cells stained with the chimeric rabbit IgG version of TOC1 (orb1463352) (1:1000 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1500 dilution), showing nuclear staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb1463352, DAPI, merged channels and an isotype control. The isotype control was anti-fluorescein antibody (orb256390) followed by staining with Alexa Fluor® 488 secondary antibody.

Western Blot using anti-Tau Oligomeric complex antibody TOC1. Kelly(A) (0.5 µg/ml) and U251(B) (0.5 µg/ml) cells lysate (35 µg protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of TOC1 (orb1463352) before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.