SOCS3 Antibody

• Catalog Number: orb214849
• Category: Antibodies
• Description: The SOCS3 Antibody is suitable for IF, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the center region of human SOCS3. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Bovine, Canine, Gallus, Human, Mouse, Porcine, Rat, Sheep
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human SOCS3. The exact sequence is proprietary.
• UniProt ID: O88583, O14543, O35718
• Tested applications: IF, WB
• Dilution range: WB: 1-500-1-1000
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CIS3; SSI3; Suppressor of cytokine signaling 3; SO
• Research Area: Epigenetics
• Note: For research use only
• Entrez: 89829, 9021, 12702
• Expiration Date: 12 months from date of receipt.

Western blot analysis of SOCS3 expression in U87MG (A), HEK293T (B) whole cell lysates. (Predicted band size: 24 kD; Observed band size: 24 kD)

Immunofluorescent analysis of SOCS3 staining in A2780 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).