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Catalog Number | orb499629 |
---|---|
Category | Antibodies |
Description | SOCS3 Rabbit Polyclonal Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, IF, IHC-Fr, IHC-P |
Predicted Reactivity | Bovine, Canine, Equine, Gallus, Mouse, Porcine |
Reactivity | Human, Rat |
Isotype | IgG |
Immunogen | KLH conjugated synthetic peptide derived from human SOCS3 1/80/225 |
Antibody Type | Primary Antibody |
Concentration | 1mg/ml |
Dilution range | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 25 kDa |
Target | SOCS3 |
UniProt ID | O14543 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
Alternative names | suppressor of cytokine signaling 3; ATOD4; CIS 3; Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Blank control (blue line): Hep G2 (fixed with 70% ethanol (Overnight at 4°C) and then permeabilized with 90% methanol for 20 min at -20°C). Primary Antibody (green line): Rabbit Anti-SOCS3 antibody (orb499629), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, dilution: 1 µg/Test.
Paraformaldehyde-fixed, paraffin embedded (Rat skeletal muscle), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (SOCS3) Polyclonal Antibody, Unconjugated (orb499629) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
FC, WB | |
Bovine, Canine, Equine, Porcine, Rabbit, Sheep | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, IF | |
Bovine, Canine, Equine, Gallus, Mouse, Porcine | |
Human, Rat | |
Rabbit | |
Polyclonal | |
PE |
IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC, IP, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, WB | |
Human, Mouse, Porcine, Rat, Sheep | |
Rabbit | |
Polyclonal | |
Unconjugated |