Smad4 Rabbit Polyclonal Antibody

SKU: orb251580

Description

Anti-Smad4 Antibody. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Research Area

Cancer Biology, Cardiovascular Research, Cell Biology, Epigenetics & Chromatin, Signal Transduction, Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: WB
• Dilution Range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Smad4, identical to the related mouse and rat sequences.
• Target: Mothers against decapentaplegic homolog 4
• Molecular Weight: 60 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Mothers against decapentaplegic homolog 4; MAD homolog 4; Mothers against DPP homolog 4; Deletion target in pancreatic carcinoma 4; SMAD family member 4; SMAD 4; Smad4; hSMAD4; SMAD4; DPC4, MADH4

Anti-SMAD4 Picoband antibody, Western blotting. All lanes: Anti SMAD4 at 0.5 ug/ml. Lane 1: Rat Brain Tissue Lysate at 50 ug. Lane 2: Mouse Brain Tissue Lysate at 50 ug. Lane 3: Rat Skeletal Muscle Tissue Lysate at 50 ug. Lane 4: Mouse Skeletal Muscle Tissue Lysate at 50 ug. Lane 5: U87 Whole Cell Lysate at 40 ug. Lane 6: Human Placenta Tissue Lysate at 50 ug. Lane 7: HT1080 Whole Cell Lysate at 40 ug. Lane 8: HELA Whole Cell Lysate at 40 ug. Lane 9: NEURO Whole Cell Lysate at 40 ug. Predicted bind size: 60 KD. Observed bind size: 60 KD.

Quick Database Links

Gene Symbol

Mothers against decapentaplegic homolog 4

UniProt

Q13485