SMAD1 Rabbit Polyclonal Antibody

SKU: orb251578

Description

Anti-SMAD1 Antibody. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Research Area

Cancer Biology, Cell Biology, Epigenetics & Chromatin, Metabolism Research, Signal Transduction, Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: WB
• Dilution Range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human SMAD1, different from the related mouse sequence by two amino acids, and from the related rat sequence by five amino acids.
• Target: Mothers against decapentaplegic homolog 1
• Molecular Weight: 52 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Mothers against decapentaplegic homolog 1; MAD homolog 1; Mothers against DPP homolog 1; JV4-1; Mad-related protein 1; SMAD family member 1; SMAD 1; Smad1; hSMAD1; Transforming growth factor-beta-signaling protein 1; BSP-1; SMAD1; BSP1, MADH1, MADR1

Anti-SMAD1 Picoband antibody, Western blotting. All lanes: Anti SMAD1 at 0.5 ug/ml. Lane 1: Rat Cardiac Muscle Tissue Lysate at 50 ug. Lane 2: Mouse Cardiac Muscle Tissue Lysate at 50 ug. Lane 3: Rat Skeletal Muscle Tissue Lysate at 50 ug. Lane 4: Mouse Skeletal Muscle Tissue Lysate at 50 ug. Lane 5: 293T Whole Cell Lysate at 40 ug. Lane 6: MCF-7 Whole Cell Lysate at 40 ug. Lane 7: HELA Whole Cell Lysate at 40 ug. Predicted bind size: 52 KD. Observed bind size: 52 KD.

Quick Database Links

Gene Symbol

Mothers against decapentaplegic homolog 1

UniProt

Q15797