Smad1 (phospho Ser465) rabbit pAb

• Catalog Number: orb764332
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Smad1 (phospho-Ser465)
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Immunogen: The antiserum was produced against synthesized peptide derived from human Smad1 around the phosphorylation site of Ser465. AA range:416-465
• MW: 60
• Tested applications: ELISA, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-SMAD1 antibody, anti-BSP1 antibody, anti-MADH
• Note: For research use only

Western Blot analysis of various cells using Phospho-Smad1 (S465) Polyclonal Antibody.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Smad1 (Phospho-Ser465) Antibody.

Immunohistochemistry analysis of paraffin-embedded human heart, using Smad1 (Phospho-Ser465) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from HeLa cells treated with Serum 10% 15', using Smad1 (Phospho-Ser465) Antibody. The lane on the right is blocked with the phospho peptide.