Anti-SMAD1 Antibody

• Catalog Number: orb1473770
• Category: Antibodies
• Description: Mouse monoclonal antibody to SMAD1
• Target: SMAD1
• Clonality: Monoclonal
• Species/Host: Mouse
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Buffer/Preservatives: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: This antibody is purified through a protein G column.
• Immunogen: Recombinant fusion protein of human SMAD1. The exact sequence is proprietary.
• UniProt ID: Q15797
• Tested applications: IF, IH, WB
• Dilution range: WB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/10 - 1/50)
• Antibody Type: Primary Antibody
• Source: Mouse
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: BSP1; MADH1; MADR1; Mothers against decapentaplegi
• Note: For research use only
• Entrez: 4086

Western blot analysis of SMAD1 expression in HT1080 (A), Hela (B), 293 (C) whole cell lysates. (Predicted band size: 52 kD; Observed band size: 60 kD)

Immunohistochemical analysis of SMAD1 staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of SMAD1 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF555 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).