Anti-SLC25A11 Antibody

• Catalog Number: orb214782
• Category: Antibodies
• Description: Rabbit polyclonal antibody to SLC25A11
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Primate, Rat
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human SLC25A11. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Dilution range: WB: 1-500-1-1000
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: SLC25A11
• Entrez: 64201, 8402, 67863
• UniProt ID: Q9CR62, Q02978, P97700
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti SLC20A4 antibody, anti Mitochondrial 2-oxoglu
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of SLC25A11 expression in HEK293T (A), Hela (B), rat kidney (C), rat spleen (D) whole cell lysates. (Predicted band size: 34 kD; Observed band size: 34; 28 kD)

Immunofluorescent analysis of SLC25A11 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).